VT-iSIM is the worlds first truly high speed super resolution live cell imaging system
Super Resolution
Whilst maintaining the necessary temporal resolution and at a low photobleaching level, VT-iSIM offers a spatial resolution of 100nm and axial resolution of 300nm
Photo courtesy of Prof. Dr. Peter Lipp, Universität des Saarlandes, Center for Molecular Signaling (PZMS)
Live Cell Imaging
Generates super-resolution images in real-time and in a low phototoxicity environment, enabling live-cell imaging capability
Time Series courtesy of Quantitative Imaging: From Acquisition to Analysis Course, CSHL
High Temporal Resolution
Capable of observing dynamic events by producing super-resolution images up to 200 fps at a pixel resolution of 1500×1024
Photo courtesy of Prof. Dr. Peter Lipp, Universität des Saarlandes, Center for Molecular Signaling (PZMS)
Exceptional Imaging Depth
Can image > 10x deeper than traditional SIM
Photo courtesy of Rusan Lab,NIH (NHLBI)
High Signal to Noise
The combination of multi-point scanning and VisiTech’ patented Ingwaz scanning architecture, VT-iSIM offers very low photo-bleaching and very high signal to noise
Photo courtesy of Kops Lab, Hubrecht Institute, The Netherlands
Highly Configurable
Highly configurable camera-based system, can be integrated across multiple imaging platforms. Find out more on the System Integration page.
Specifications
Spatial Resolution
100nm Laterally and 300nm Axially*
Temporal Resolution
Scan Speed up to 1000fps
Pin holes
Selectable from 10-64µm
Emission Filter Changer
Regular or High Speed 6-Position Emission Filter Changer
Excitation
Up to 6 solid state lasers range from 400nm to 700nm
Sync
Perfect camera & excitation sync comes as standard
Camera Specification
For accurate sampling camera must have pixel size <6.5um, Camera connection is via regular c-mount
Microscope Specification
Nikon, Olympus, Zeiss, Leica. Connection via regular c-mount
Software
Visi-View, Cell Sense, Micro-Manager, Meta-Morph & NIS Elements
* Spatial resolution measured using PSFj with Nikon 100x 1.49NA Lens, contact VTi for more details